Prostaglandins F in uterine tissue and venous plasma of ewes with intrauterine devices.

نویسندگان

  • J E Pexton
  • S P Ford
  • L Wilson
  • R L Butcher
  • E K Inskeep
چکیده

Levels of prostaglandins F (PGF) in uterine tissues and in uterine and peripheral plasma from ewes with and without IUD's were examined. Mature ewes were laparotomized on day 2 of the estrous cycle. Fifteen ewes with a corpus luteum on only one ovary were assigned at random to either a treated group (10 ewes) which received an intrauterine device (IUD) in each uterine horn or to a control group (five ewes). Twelve ewes had a corpus luteum on each ovary and received at random an IUD in one uterine horn (eight ewes) or a sham operation (four ewes). Blood was collected from a major vein of each uterine horn on day 5. Uteri were removed and the uterine horns were separated and dissected into caruncular and intercaruncular endometrium and myometrium. Prostaglandins F were extracted with ethyl acetate, separated by column chromatography on silicic acid and quantified by radioimmunoassay. An IUD in one or in both uterine horns resulted in higher concentrations (ng/ml) of PGF in uterine venous plasma from both horns

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Prostaglandins F in uterine venous plasma, ovarian arterial and venous plasma and in ovarian and luteal tissue of pregnant and nonpregnant ewes.

Ovarian venous, ovarian arterial and uterine venous blood were collected from the same side as a corpus luteum in 10 nonpregnant and 10 pregnant ewes at day 15 postestrus. Both ovarian and uterine venous samples were collected by venipuncture. Ovarian arterial blood was collected into heparinized centrifuge tubes after a small portion of the artery was dissected free from mesovarium and then se...

متن کامل

Effect of continuous intrauterine administration of prostaglandin E2 on life span of corpora lutea of nonpregnant ewes.

The antiluteolytic effect of prostaglandin (PG) E2 was tested in two experiments. In experiment I, seven nonpregnant ewes that had ovulated bilaterally, were laparotomized on day-11 postestrus. Uterine horns were isolated by ligatures between the internal and external bifurcations and around blood vessels in the intercornual area. An Alzet TM minipump (25 • 6.5 ram) containing PGE2 (delivery ra...

متن کامل

Uterine and peripheral plasma F-prostaglandins correlated with peripheral progesterone in cyclic rats.

Hadioimmunoassay (RIA) measurements of F-prostaglandins (PGF) were made in uterine venous and peripheral venous plasma. RIA determinations of progesterone (P) were also made in the peripheral venous plasma from the same rats. Concentrations of PGF in uterine venous plasma were lowest between 1000-1200 h of estrus (6.07 ng/ml), peaked at metestrus between 1000-1200 h (12.50 ng/ml), decreased on ...

متن کامل

Platelet-activating factor may act as an endogenous pulse generator for sheep of luteolytic PGF2a release

Chami, O., A. Megevand, T. Ott, F. Bazer, and C. O’Neill. Platelet-activating factor may act as an endogenous pulse generator for sheep of luteolytic PGF2a release. Am. J. Physiol. 276 (Endocrinol. Metab. 39): E783–E792, 1999.— Pulsatile release of uterine prostaglandin F2a (PGF2a) induces luteolysis in ruminants. However, the mechanism(s) that initiates and maintains luteolysis has not been de...

متن کامل

Platelet-activating factor may act as an endogenous pulse generator for sheep of luteolytic PGF2αrelease.

Pulsatile release of uterine prostaglandin F2α(PGF2α) induces luteolysis in ruminants. However, the mechanism(s) that initiates and maintains luteolysis has not been defined. The present study tested the hypothesis that the endogenous PGF2α pulse generator is uterine-derived platelet-activating factor (PAF). Ovariectomized ewes were given exogenous progesterone (P), estradiol (E), or both (P+E,...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of animal science

دوره 41 1  شماره 

صفحات  -

تاریخ انتشار 1975